시설(장비)예약

시설(장비)소개

400 MHz 핵자기공명분광기

400 MHz Nuclear Magnetic Resonance Spectrometer400 MHz 핵자기공명분광기

  • 모델명AVANCE Neo 400 Nanobay
  • 제조사BRUKER
  • 설치장소한양맞춤의약연구원 103호
  • 담당자이영복 교수님 031-400-5500

첨단 의약-바이오 산업, 에너지 산업, 전자정보기기 산업 및 NT-BT 융합기술의 폭발적인 발전에 따른 의약품, 고분자, 신소재, 베터리, 촉매, 및 유무기 화합물 등의 시료 분석 및 연구에 대한 수요 충족

- 다양한 유무기 소재들의 3차원 구조분석 및 정성 / 정량 분석에 활용

- 단백질, DNA, RNA와 같은 거대분자들의 구조결정에 활용

- 분자들의 Dynamics 변화 연구

- 점성도가 높은 시료들의 고온 구조 분석 연구

- 유기 고분자 및 고체 무기물 정성 / 정량 분석에 활용

- 고온 초전도체와 반도체 성분 분석

 

 

- 다양한 제약 및 생체 조직 분석

. Magnet System1 Sys

(1) Actively shielded superconducting magnet : 54mm or more bore size

(2) Magnet field strength : 9.4 Tesla or more

(3) L-He hold time : 300 days or more

(4) Magnet stray fields : 5 gauss or more line from magnet center

- Horizontal distance : 0.5 m or less

- Vertical distance : 1.0 m or less

(5) Shim system : 36 orthogonal shims or more

(6) Anti-vibration damping system : frequency damped 3.8 Hz or less

(7) External disturbance suppression : 99% or more

2. Spectrometer Console1 Sys

(1) Frequency channels : Fully broadband frequency generation with three numerically controlled oscillators for three channels

1) Channel number : 2 channels or more

2) Frequency range

a) Channel 1 : 5~1280 MHz or wider

b) Channel 2 : 5~1280 MHz or wider

3) Frequency resolution : 0.005 Hz or less

4) Phase resolution : 0.01° or less

5) Amplitude resolution : 0.1 dB or less

6) Amplitude control (attenuation) : 90 dB or more

7) Waveform memory : 1 GB or more

(2) Digital receiver system

1) Two or more receiver unit

2) Effective dynamic range of digitizer : 23 bit or more

(3) Amplifier system requirements

1) Amplifier #1

a) Max. pulse power : 50 Watt or more

b) Frequency range [1H/19F] : 180~400 MHz or wider

2) Amplifier #2

a) Max. pulse power : 140 Watt or more

b) Frequency range : 41~162 MHz or wider

(4) Intermediate frequency (IF) : 1,852 GHz or more

(5) Waveform memory : 1 GB or more

(6) Timing resolution : 12.5 nanosec or less

(7) Spectral width : 7.5MHz or more

 

3. Computer System & Softwares1 Set

(1) Computer : 3.8GHz or more Quad Core CPU

(2) Memory: 16 GB RAM or more

(3) Disk : 2 TB HDD or more

(4) Operating system : Windows 10 Pro 64-bit English or more

(5) 24" TFT monitor or more

(6) NMR data acquisition (arbitrary dimensions) and processing (1D, 2D, 3D,

4D and 5D)

(7) Menu guided acquisition setup

(8) NMR automation interface

(9) Structure Analysis Software

1) Relaxation analysis (T1/T2)

2) Integration of 1D and 2D spectra

3) Deconvolution of 1D and 2D spectra

4) Experiment simulation

5) Spectrum simulation

6) Interactive and automatic multiplet analysis

7) Solid state lineshape analysis

8) Integrated structure editor

9) Integrated solid state NMR set-up

10) Supporting NMR Data Interpretation

11) Non-Uniform sampled (NUS) data for 2D spectra

12) Smart data acquisition

 

4. Probehead

(1) 5mm X-nuclei double resonance broadband cryoprobe 1 Set

1) 1H or 19F decoupling or observation

2) 2H lock

3) Observe nuclei : 1H,19F and BB range=31P-15N excluding 199Hg-153Eu

4) Actively shielded z-gradient coil

5) Automatic tuning and matching for all nuclei

6) VT range : -40oC to +150oC or wider

(2) 5mm X-nuclei double resonance broadband probe1 Set

1) 1H decoupling and observation

2) 2H lock

3) Observe nuclei : 1H,19F and BB range=31P-109Ag excluding 171Yb-9Be

4) Actively shielded z-gradient coil

5) Automatic tuning and matching for all nuclei

6) VT range : -150oC to +150oC or wider

 

5. Auto sample changer1 Set

(1) Convenient sample access at user height

(2) Safe operation

(3) 24 or more easily accessed sample positions

(4) Random access for sequential or batch automation

(5) Unique manual push-button sample exchange

(6) Supports all common sample tubes in spinners or shuttles

 

6. Accessories

(1) N2 Cryo-Platform for N2 cryoprobe 1 set

(2) Balance to measure N2 filing level 1 set

(3) Nitrogen gas separator1 set

(4) 5mm spinners24 ea

(5) LaserJet printer1 set

(6) UPS KVA, 30min. back-up time 1 set

(7) Aircompressor

(8) Air dryer1 set

(9) LHe & LN2 for installation 1 set

(10) VT gas cooler for VT gas cooling, 40oC 1 set

 

 

(11) Mnova NMR processing software license 2 ea

- NMR 장비와 연결된 컴퓨터에 사용자 로그인을 한 후 Topspin 프로그램을 구동한다.
- 측정하고자하는 원자핵 (1H, 13C, 등) 실험을 셋팅한 후 준비된 NMR tube를 NMR 장비에 올바르게 넣는다. 


- 중수소치환된 용매 (D2O, CDCl3, 등) 500 uL 정도에 소량의 시료를 넣은 후 혼합용액을 만든다.
- 혼합용액을 외경이 5 mm인 NMR tube에 넣은 후 spinner에 연결한다.